Alligator Blood may have run his last race, with trainers Gai Waterhouse and Adrian Bott confirming the weight-for-age star galloper has suffered a serious injury.

A winner of seven Group 1 races, Alligator Blood injured a pastern during a routine piece of trackwork on Saturday morning.

The popular seven-year-old had won a Sydney barrier trial 48 hours earlier.

A pastern injury is a less common issue than a fetlock or tendon problem that needs nine-to-12 months to heal.

Waterhouse confirmed Alligator Blood will need a year off racing for the injury to be rehabilitated.

"He will be out for a year and we are still hoping he may come back and race again," Waterhouse told News Corp.

Alligator Blood already has a comeback story after he was diagnosed with a 'kissing spine' in late 2020.

He spent an extended term out of racing before eventually coming under the care of Waterhouse and Bott, who were able to get the best out of him.

"Everyone is sad, he is such a special horse. I have probably only ever had one or two horses this good," Waterhouse said.

"Most horses never win a Group 1 and to win that many, it's just magical.

"He will have a lot of rehab in that time then hopefully he will come back to take on the best of the best. He will be monitored throughout very closely."

"I don't know if he will race again, but one thing I do know, for racing fans, is that he won't be seen again until 2025."

Alligator Blood collected spring Group 1 wins in the Underwood Stakes and Caulfield Stakes before a minor placing in the Cox Plate.

He completed his 2023 campaign when third in the Champions Mile during the Melbourne Cup carnival.